RNA is sufficiently released from blood cells by modified lysis buffer after selective precipitation of protein, hemoglobin by a unique buffer and removal of residual DNA through filtration. Total RNA is obtained by a column purification as long as the addition of isopropanol. Therefore a high recovery yield of good quality RNA will be selectively obtained in this way. A high integrity with an excellent purity of RNA by the miniprep kit will be completely applicable to relevant molecular experiments, such as Northern blot, RT-PCR, translation in vitro, primer extension, S1 nuclease mapping, RNase protection and cDNA library construction, etc.
